The "activated" hepatic glucocorticoid-receptor complex. Its generation and properties.
نویسندگان
چکیده
منابع مشابه
The Purified Activated Glucocorticoid Receptor
The structure of purified preparations of activated (DNA-binding) glucocorticoid receptor (GR) was analyzed in the presence or absence of DNA. A 35-base pair DNA fragment harboring a strong GR-binding site from the mouse mammary tumor virus promoter (-189/-166) was used for stoichiometric analysis of the GR . DNA complex. Glycerol gradient centrifugation was utilized in order to separate the 6 ...
متن کاملEffect of pyridoxal phosphate on the DNA binding site of activated hepatic glucocorticoid receptor.
The binding of rat liver glucocorticoid.receptor complexes to DNA-cellulose and nuclei has been studied after activation of the complexes by heating. Subsequent exposure to pyridoxal 5'-phosphate or pyridoxal markedly inhibited this binding. In one system 0.75 mM pyridoxal 5'-phosphate or 6.5 mM pyridoxal gave 50% inhibition. Pyridoxamine 5'-phosphate, pyridoxamine, and pyridoxine did not inhib...
متن کاملRole of chemical reagents in the activation of rat hepatic glucocorticoid-receptor complex.
The effect of sulfhydryl modifying reagents on the activation of hepatic glucocorticoid-receptor complex was studied. Unactivated (preincubated at O O C ) r3H]dexamethasone-receptor complex pretreated with Nethylmaleimide or iodoacetamide at 0°C and then treated at 25°C was unable to bind to rat liver nuclei. On the other hand, [3HJdexamethasone-receptor complex first incubated at 25OC an...
متن کاملThe Importance of α-CT and Salt bridges in the Formation of Insulin and its Receptor Complex by Computational Simulation
Insulin hormone is an important part of the endocrine system. It contains two polypeptide chains and plays a pivotal role in regulating carbohydrate metabolism. Insulin receptors (IR) located on cell surface interacts with insulin to control the intake of glucose. Although several studies have tried to clarify the interaction between insulin and its receptor, the mechanism of this interaction r...
متن کاملThe Importance of α-CT and Salt bridges in the Formation of Insulin and its Receptor Complex by Computational Simulation
Insulin hormone is an important part of the endocrine system. It contains two polypeptide chains and plays a pivotal role in regulating carbohydrate metabolism. Insulin receptors (IR) located on cell surface interacts with insulin to control the intake of glucose. Although several studies have tried to clarify the interaction between insulin and its receptor, the mechanism of this interaction r...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41893-0